Table 1. Specifications of the five reference genes under evaluation.

Reference Gene/Alias	HUGO gene abbreviation	RNA class	Chromosome Location	NCBI reference	References
5S; r5S	RN5S1@	Ribosomal RNA (rRNA)	1q42.11-q42.13	V00589.1	Zhang Y et al.41
hsa-miR-16-5p; miR-16	MIR16-1	microRNA (miRNA)	13q14.2	LM378756.1	Nishi H et al.29 Roy S et al.42
SNORD44 U44 RNU44	SNORD44	Small nucleolar RNA (snoRNA)	1q25.1	NR_002750.2	Ferreira LR et al.43
SNORD48; U48; RNU48	SNORD48	Small nucleolar RNA (snoRNA)	6p21.33	NR_002745	Sauer E et al.19
U6; RNU6	Not available from the provider	Small nuclear RNA (snRNA)	Not available from the provider	Not available from the provider	Satoh M et al.44 Cooley N et al.28Adam O et al.45 Villar AV et al.46García R et al.47 Song CL et al.48Liu H et al.32 Dong S et al.49