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. 2017 Jan 24;7:41122. doi: 10.1038/srep41122

Figure 8. No Sox9 positive cells express the astrocyte marker Aldh1l1 in the absence of GFAP.

Figure 8

(a) Schematic of SCI crush lesion with boxed region of lateral portion of astrocyte scar (AS) shown in b,c. (b,c) Single channel and merged immunofluorescence images showing complete overlap of staining for Sox9, GFAP and Aldh1l1 in a horizontal section through the lateral astrocyte scar. (d) Schematic of SCI crush lesion showing the 4 counting boxes evaluated in the lateral portions of the astrocyte scar. Graphs show the mean number of cells per volume and mean percent of total Sox9 or total GFAP labeled cells that are co-labeled with Sox9, GFAP or Aldh1l1 in the lateral astrocyte scar. n = 6 per group, *p < 0.001 versus GFAP + Aldh1l1 + Sox9 (ANOVA with Newman-Keuls), ^p < 0.001 (t-test). (e–g) Single channel and merged immunofluorescence images showing Sox9, GFAP and Aldh1l1 in a horizontal section through the ependymal layer (Ep), lesion core (LC) and astrocyte scar. (f) Schematic of SCI crush lesion with boxed region of ependyma and astrocyte scar shown in (g,h). (g) Higher magnification of boxed area in e, showing ependymal cells positive for Sox9 only and astrocytes positive for Sox9, GFAP and Aldh1l1. (h) Higher magnification of boxed area in g. Note in (e-g) that all cells positive for Sox9 and Aldh1l1 are also positive for GFAP. (i) Schematic of SCI crush lesion showing the 4 counting boxes evaluated in the medial portions of the astrocyte scar adjacent to and including the ependyma. Graphs show the mean number of cells per volume and mean percent of total Sox9 or total GFAP labeled cells that are co-labeled with Sox9, GFAP or Aldh1l1 in the lateral astrocyte scar. n = 6 per group, *p < 0.01, **p < 0.001 versus GFAP + Aldh1l1 + Sox9 (ANOVA with Newman-Keuls), ^p < 0.01, ^^p < 0.001 (t-test).