Table 3. Emulsification activity of 2% (w/v) EPS solutions from Acidobacteria strains WH15 (EPSWH15) and 5B5 (EPS5B5), Tween 80 and xanthan with different oils and different hydrocarbons in 3:2 ratio (v/v).
Oil/Hydrocarbon | Positive Controlsa |
Acidobacterial Exopolysaccharidesa |
||||||||
---|---|---|---|---|---|---|---|---|---|---|
Tween 80 | Xanthan |
EPSWH15 |
EPS5B5 |
|||||||
E24 | E24 | E168 | E360 | E24 | E168 | E360 | E24 | E168 | E360 | |
Sunflower seed oil | 100 ± 0.02a* | 50.03 ± 2.43a | 44.70 ± 2.40 | 30.56 ± 2.78 | 66.98 ± 1.45a | 63.00 ± 2.41 | 62.04 ± 1.60 | 67.89 ± 4.18a | 64.57 ± 1.85 | 66.65 ± 0.93 |
Diesel oil | 63.21 ± 2.96b | 76.85 ± 4.24b | 67.84 ± 8.24 | 66.02 ± 6.67 | 12.28 ± 1.75b | 12.28 ± 1.75 | 14.43 ± 2.98 | 33.65 ± 2.83b | 33.65 ± 2.83 | 32.45 ± 4.08 |
Liquid paraffin oil | 91.35 ± 3.42a | 70.81 ± 12.36ab | 39.00 ± 3.23 | 37.12 ± 4.46 | 58.71 ± 0.65c | 49.50 ± 2.95 | 54.13 ± 1.39 | 54.18 ± 3.66a | 52.78 ± 2.78 | 51.68 ± 9.52 |
Toluene | 46 ± 1.54bc | 38.26 ± 7.88ac | 29.10 ± 1.85 | 9.08 ± 1.50 | 10.19 ± 1.60b | 10.29 ± 1.70 | 11.17 ± 2.56 | 21.30 ± 1.60b | 21.48 ± 1.28 | 21.30 ± 1.60 |
Hexane | 49 ± 2.45bc | 22.51 ± 12.42c | 23.15 ± 1.60 | 7.41 ± 1.60 | 26.85 ± 1.60d | 26.85 ± 1.60 | 25.93 ± 1.16 | 59.26 ± 8.93a | 58.33 ± 4.81 | 50.92 ± 4.24 |
aResults are expressed as percentages of the total height occupied by the emulsion; Values in bold: Emulsifier Index (E24) with values above 50%; Mean values of three replicates ( ± standard deviation). *Treatments with the same letter do not differ by the by the Tukey’s post hoc test (P < 0.05).
E24 emulsification index after 24 h; E168 emulsification index after 168 h; E360 emulsification index after 360 h.