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. 2017 Jan 24;7:41123. doi: 10.1038/srep41123

Figure 1. NLRP3 inflammasome activation in IgA ICs-primed macrophages.

Figure 1

NLRP3 and pro-IL-1β levels by Western blotting after incubated for 6 h with IgA ICs and semi-quantitative analysis in J774A.1 macrophages (a). Caspase-1 levels by Western blotting and semi-quantitative analysis (b), and IL-1β levels by Western blotting and semi-quantitative analysis and ELISA (c) in J774A.1 macrophages were incubated for 5.5 h with IgA ICs and 30 min ATP. IL-1β secretion or caspase-1 activity measured by ELISA in shSC or shNLRP3 J774A.1 macrophages and/or primary peritoneal macrophages from untreated wild type or NLRP3 KO mice, which were incubated for 5.5 h with IgA ICs and 30 min ATP (d). Caspase-11 mRNA levels in cell lysate measured by real-time PCR (e) and IL-1β secretion by ELISA (f) in shSC or shcaspase-11 J774A.1 macrophages incubated for 5.5 h with IgA ICs and 30 min ATP. The data are expressed as the mean ± SEM for three separate experiments. *p < 0.05. WT, wild type. IgA ICs, IgA immune complexes. ns, no significant difference.