Figure 4. Deletion of Rer1 leads to axonal swellings and degeneration of Purkinje cells.

(a) Cerebellar slices of mice of indicated age and phenotype were immunostained for calbindin (calb, red) and GFAP (green) and analyzed by confocal microscopy. Single confocal sections are shown. (b) Cerebellar slices from P36, 4 and 9 months (mo) old Rer1^ΔPC mice and control littermates were processed for calbindin immunofluorescence and analyzed by confocal microscopy. Shown are merges of three confocal sections. Arrowheads indicate intact axons of PCs, arrows axonal swellings. Scalebar 20 μm.