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. 2017 Jan 24;7:41226. doi: 10.1038/srep41226

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Representative images of the endolysosomal calcium release of HepG2 detected by Fluo8-AM calcium indicator at 1 s, 60 s, 90 s, 330 s, 390 s, and 420 s after incubation of 10 μM tamoxifen, clomiphene or U18666a for 1 h. (b) The calcium release of HepG2 loaded with 1 μM TG at 60 s for 30 s and 200 μM GPN at 330 s for 60 s. HepG2 cells were pre-treated with 10 μM tamoxifen, clomiphene or U18666a for 1 h. (c) The quantification of calcium release induced by 200 μM GPN. Data are expressed as the means ± SEM (n > 20). Significant differences versus control group are presented by asterisks (*), ***P < 0.001.