Figure 5.

Rate constants of catalyzed reactions between the catalyst (1 nM), fuel (13 nM), substrate (14 nM), and reporter (20 nM) monitored via fluorescence. The black bar represents the original fuel strand. Pink, orange, and blue bars represent 1 and 2 nt modifications at 5′, nick, and 3′ locations, respectively. Error bars show the standard deviation from the mean for select samples in triplicate to estimate experimental error.