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| ID. of TG F0 mice (gender) | Copy number of transgene in TG F0 mice | Number of tested F1 mice (litter number) | Number of TG F1 mice (positive rate) |
|---|---|---|---|
| 551 (male) | 1 | 29 (5) | 15 (51.7%) |
| 553 (male) | 1 | 34 (5) | 16 (47.1%) |
| 559 (female) | 5 | 17 (2) | 15 (88.2%) |
| 560 (female) | 4–5 | 16 (2) | 14 (87.5%) |
Copy number of transgene in TG F0 mice was determined by Southern blot as shown in Fig. 1D. Number of TG F1 mice was determined by PCR analysis.
