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. 2017 Jan 24;7:41046. doi: 10.1038/srep41046

Figure 2. Clenbuterol mitigates the atrophy induced by polyglutamine-expanded AR in C2C12 myotubes.

Figure 2

(A) Upper panels, representative bright-field images of C2C12 myotubes expressing AR24Q and AR100Q treated with vehicle, DHT (10 nM), and clenbuterol (clenb, 10 μM) for 14 DIV. Bottom panel, myotube width analysis. Graph, mean ± SEM, n = 3 independent experiments. Two-way ANOVA + SNK. NS, non-significant. Bar, 25 μm. (B,C) Western blotting analysis of phosphorylated and total Akt (B) and CREB (C) in C2C12 myotubes expressing AR24Q and AR100Q and cultured as in (A). Phosphorylated and total Akt and CREB were detected with specific antibodies, and calnexin (CNX) was used as loading control. Graph, mean ± SEM, n = 6 (B) and 5 (C) independent experiments. Two-way ANOVA + SNK.