Figure 6. Ribo-Zero rRNA depletion improves transcript detection by qPCR.

(A–C) qPCR threshold cycle (C_q) for the indicated rRNA transcripts. (D–F) Copy numbers of mreB, brlR, and PA0701 transcripts were calculated following the establishment of respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of total RNA rRNA-depleted RNA processed using the indicated kits. qPCR was also performed on cDNA generated from 1 μg of total RNA, with this sample used a control representing standard qPCR conditions. Experiments were repeated using three biological replicates. Error bars indicate standard deviation.