Figure 2. 3D-simulation and mass spectrometry based identification of representative protein spots.

(A) Upper panel shows the image of encircled 2-D gel spots in healthy and CP-CML subjects while the lower panel shows their corresponding 3D-images obtained using Image Master 2D-Platinum software; difference in spot intensities amongst the two study subjects are clearly visible. (B) Peptide mass fingerprint data of selected protein spot obtained following MALDI-TOF/TOF mass spectrometry analysis. (C) Identification of protein using online MASCOT program; matched peptide sequences of identified protein having sequence coverage of 55%, are shown in red bold.