. 2017 Jan 24;7:40943. doi: 10.1038/srep40943

Table 3. List of proteins identified in the plasma samples of controls and the CP-CML subjects by MALDI-TOF MS.

Sr. No	Protein Name	Accession Number	Spot No.	Expression	p-value	FDR^*	pI		Approx. MW (kDa)		Mascot Score	Sequence Coverage (%)	Mass values searchd	Mass values matched	PMF Score^**
Sr. No	Protein Name	Accession Number	Spot No.	Expression	p-value	FDR^*	Observed	In Database	Observed	In Database	Mascot Score	Sequence Coverage (%)	Mass values searchd	Mass values matched	PMF Score^**
1	α-1-antichymotrypsin	gi\|1340142	L1a-gN1a-d	2.96 ± 1.060.84 ± 0.50	0.021	0.05	4.53–4.84	5.34	71–76	47.79	64	25	68	9	205.15
2	α-1-antitrypsin	gi\|177831	L2a-dN2a-c	10.04 ± 4.1403.20 ± 2.21	0.004	0.04	5.04–5.23	5.43	62–66	46.84	210	55	93	28	255.86
3	Haptoglobin	gi\|3337390	L3a-fN3a-g	7.58 ± 3.515.91 ± 3.87	0.482	0.48	4.88–5.54	6.14	44–52	38.72	110	35	71	14	163.25
4	Chain C, Human complement component C3c	gi\|78101271	L4N4a,b	0.93 ± 0.310.84 ± 0.62	0.102	0.15	4.96	4.79	45	40.20	107	40	63	14	138.28
5	α-1-β glycoprotein	gi\|119592981	L5a-dN5a-d	0.93 ± 0.250.61 ± 0.22	0.165	0.19	5.21–5.41	5.58	79–81	54.81	70	22	67	10	126.96
6	Vitamin D-binding protein isoform 1 precursor	gi\|32483410	L6a-cN6a-d	0.78 ± 0.161.21 ± 0.27	0.021	0.05	5.38–5.54	5.32	61–62	54.48	113	28	70	15	146.65
7	Antithrombin III variant	gi\|576554	L7a-cN7a-e	1.98 ± 1.223.53 ± 2.77	0.177	0.19	5.30–5.45	6.11	70–71	53.11	69	25	39	11	121.47
8	Hemopexin precursor	gi\|11321561	L8a-dN8	2.65 ± 1.211.20 ± 0.94	0.049	0.08	5.42–5.67	6.55	80–85	52.39	106	34	49	16	140.5
9	Fibrinogen γ	gi\|223170	L9a-dN9	4.85 ± 0.792.71 ± 1.70	0.025	0.09	5.48–5.79	5.54	54–56	46.82	134	31	45	15	147.81
10	Apolipoprotein A-IV precursor, partial	gi\|178779	L10N10	0.16 ± 0.170.04 ± 0.04	0.102	0.24	5.32	5.22	48	43.36	162	53	82	22	119.78
11	Actin, β, partial	gi\|14250401	L11N11	0.16 ± 0.070.07 ± 0.10	0.205	0.36	5.48	5.56	47	41.32	100	32	74	11	128.12
12	CD5 molecule-like	gi\|5174411	L12N12	0.21 ± 0.140.02 ± 0.03	0.003	0.01	5.57	5.28	48	39.60	61	35	39	11	142.06
13	Haptoglobin isoform 2 preproprotein	gi\|186910296	L13a-cN13	0.94 ± 0.600.33 ± 0.22	0.097	0.32	5.38–5.72	6.13	43–46	38.94	73	20	26	8	108.48
14	Serum albumin	gi\|332356380	L14N14	30.58 ± 3.8629.07 ± 8.45	0.273	0.36	5.99	5.73	70	68.48	186	45	85	30	186.11
15	Apolipoprotein E	gi\|4557325	L15a, bN15	0.17 ± 0.150.15 ± 0.08	0.430	0.43	5.72–5.86	5.65	33–34	36.25	89	36	41	11	139.86
16	Chain A, crystal structure of lipid-free human apolipoprotein A-I	gi\|90108664	L16a-cN16	2.92 ± 1.102.16 ± 1.86	0.220	0.36	5.41–5.75	5.27	19–21	28.06	174	69	69	20	188.26
17	Immunoglobulin light chain	gi\|149673887	L17a-cN17a,b	9.37 ± 3.207.84 ± 1.23	0.243	0.36	6.01–6.73	6.97	22–25	23.67	80	36	47	6	79.32
18	Ras-related protein Rab-37 isoform 3	gi\|28376635	L18N18	1.79 ± 1.751.81 ± 0.36	0.252	0.36	6.37	5.97	23	24.27	67	49	45	12	108.49
19	α-2-macroglobulin, partial	gi\|177872	L19a-cN19	0.29 ± 0.170.66 ± 0.67	0.080	0.32	6.24–6.37	5.47	66–68	71.32	84	23	52	13	161.40
20	Chain B, crystal structure of human fibrinogen	gi\|237823915	L20a-cN20a-f	3.74 ± 0.882.61 ± 2.37	0.319	0.36	6.45–6.71	7.14	61–66	52.91	177	55	84	26	290.0
21	Serotransferrin	gi\|313104271	L21a-d	5.55 ± 1.836.50 ± 1.65	0.364	0.364	6.48–6.71	6.81	84–87	79.29	127	29	59	21	218.49
22	Stress-induced-phosphoprotein 1	gi\|297688341	L22a,b	0.27 ± 0.36	0.009	0.04	6.21–6.27	6.40	66–67	63.23	57	30	48	16	132.27
23	Chain C, complement C4 in complex with Masp-2	gi\|401871713	L23N23	0.35 ± 0.190.53 ± 0.16	0.055	0.09	6.68	6.37	34	33.74	82	35	34	9	128.31
24	Hap-2α/hp2-α	gi\|296653	L24a-cN24a-c	2.75 ± 2.134.79 ± 2.08	0.042	0.09	5.67–6.25	6.25	15	42.13	90	29	100	12	94.19
25	Transthyretin	gi\|114319005	L25N25	0.25 ± 0.270.83 ± 0.32	0.004	0.02	5.76	5.52	12	15.06	111	61	20	7	94.19
26	PRO2619	gi\|11493459	N26a-d	4.15 ± 6.17	0.087	0.09	6.25–6.44	5.96	60	58.51	93	23	58	12	114.16
27	ALB protein	gi\|27692693	N27a-f	3.82 ± 5.59	0.065	0.09	5.91–6.27	5.97	48–50	48.64	70	26	69	10	107.15
28	Immunoglobulin G1 Fc fragment	gi\|5031410	N28a,b	1.72 ± 2.52	0.085	0.09	6.53–6.69	6.95	31	25.40	72	43	70	9	143.82
29	Chain C, crystal structure of recombinant human fibrinogen fragment D	gi\|24987625	N29a-d	0.66 ± 1.03	0.042	0.09	5.09–6.08	5.86	26–30	35.49	79	30	66	10	105.85
30	Albumin, isoform CRA_j	gi\|119626073	N30a-e	1.96 ± 2.88	0.066	0.09	5.4–5.59	6.40	24	26.91	141	38	41	14	114.01
31	Ras-related protein Rab-3D	gi\|4759000	N31	0.73 ± 0.11	0.062	0.09	4.37	4.76	17–20	24.48	57	52	62	10	88.83
32	HPX protein	gi\|13529281	N32	0.27 ± 0.39	0.076	0.09	6.4	6.45	12	29.06	66	35	34	12	115.47
33	Fibrinogen β-chain isoform CRA_e	gi\|119625339	N33a-e	3.68 ± 2.24	0.071	0.09	5.47–5.87	6.95	40	40.16	170	59	78	24	244.39

Six proteins that qualified the three-tier criteria i.e., p-value < 0.05, FDR ≤ 0.05 and PMF score >79, are shown in bold.

^*FDR determination (the probability of expected type 1 error in null hypothesis) was performed according to the method of Diz et al.⁹. Value ≤ 0.05 indicates that 95% findings are accurate/true.

^**PMF score, for each identification, was calculated as described by Stead et al.⁷ using 79 as the cut-off value for positive hits.