Figure 1. Male kras^V12 zebrafish developed HCC faster than female during early tumorigenesis.

(A–D) Representative liver images from female (F) and male (M) wild type (wt) and kras^V12 fish (4 months old) after 10 days of doxycycline induction. The livers are outlined in dash lines. (E–H) Representative liver histology by H&E staining from fish as described in (A–D). (I) 2D liver sizes measured from the left lateral side. The liver size of wild type females (wt_F) is arbitrarily set as 1 and the sizes in other groups are relative to wt_F. (n = 15 for each group, *p < 0.05). (J) Quantification of histological phenotypes of livers from female and male kras^V12 zebrafish (**p < 0.01). (K) Fold change of kras^V12 expression in female (set as 1) and male (relative to kras_F) kras^V12 fish (NS, not significant). Scale bars, 2 mm for (A–D) and 20 μm for (E–H).