Figure 2. The male tumors were characterized by higher level of cell proliferation, β-catenin activation and E-cadherin loss.

Representative immunohistochemical staining of liver sections from female and male wild type and kras^V12 fish (4 months old) after 10 days of doxycycline induction are shown. (A–D) Immunohistochemical staining for PCNA. (E) Quantification of PCNA nuclear staining. (F–I) TUNEL staining for apoptotic cells. (J) Quantification of apoptotic cells. (K–N) Immunohistochemical staining for β-catenin. (O) Quantification of β-catenin nuclear staining. (P–S) Immunohistochemical staining for E-cadherin. (T) Quantification of cells losing E-cadherin membrane staining. *p < 0.05; **p < 0.01; ***p < 0.001; NS: not significant. Scale bar, 20 μm for all picture panels. Examples of membrane and nuclear staining are indicated by arrows and arrowheads, respectively.