Figure 6. Sex hormone treatments affected cell proliferation during liver tumor progression in kras^V12 fish.

Wild type (wt) and kras^V12 fish (4 months old) were treated with dox (doxycycline) alone, dox and E2, and dox and 11-KT, respectively, for a total of 7 days. (A–C) Representative gross observations and histology of wt and kras^V12 fish after treatment with dox (A), dox and E2 (B), dox and 11-KT (C). (D–F) Relative 2D liver size (D), quantification of tumor phenotypes observed from the liver histology (E) and quantification of the proliferating cells (%) in different female treatment groups (F). (G–I) Relative 2D liver size (G), quantification of tumot phenotypes observed from the liver histology (H) and quantification of the proliferating cells (%) in different male treatment groups (I). N = 4–5 per group for wt, n = 7–10 per group for kras^V12. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; NS: not significant. Scale bars, 4 mm for gross observation pictures and 20 μm for histology images.