Figure 3. Raman microscopic imaging of the liposomes including the chemically-activatable alkynyl steroid analogue probe before and after activation.

Microscopic images of the liposomes including 1 were obtained (a–d) before and (e–h) after incubation with TsNHNH₂. The light field images (a and e), the Raman scattering images at 1400 cm⁻¹ (b and f) and at 2120 cm⁻¹ (c and g), and their merged images (d and h) are shown. Scale bars: 5 μm. (i and j) Raman spectra obtained at the cross point on the liposome membrane in (a) and (e), respectively.