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. 2017 Jan 24;7:41007. doi: 10.1038/srep41007

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Stimulated Raman scattering (SRS) images of HeLa cells treated with chemically-activatable alkynyl steroid analogue probe and activated with TsNHNH₂ under (a–c) mildly acidic and (d–f) neutral conditions. The SRS images at (a and d) 2115 and (b and e) 2930 cm⁻¹, and (c and f) the merged images at 2115 and 2930 cm⁻¹ were obtained. The white circles and arrows indicate the areas and spots where the distinctive SRS spectra were obtained by further analysis. (g) The SRS spectra obtained from the circular areas with the number of 1 (black dotted line) and 2 (black broken line) and the spots 3 (black line) and 4 (grey line) in fig. 4c, respectively. (h) The SRS spectra obtained from the circular areas with the number of 5 (black dotted line) and 6 (black broken line) and the spot 7 (black line) in fig. 4f, respectively.