Table 1. Effect of various metabolites on SyPEPC activity.

Compounds	SyPEPC activity (in vitro)
Mock	100 ± 5.2
GTP	101 ± 0.6
Acetyl-CoA	111 ± 14.0
Fructose-1,6-bisphophate	96.9 ± 4.1
Aspartate	85.2 ± 10.7
Citrate	86.1 ± 6.7
Malate	77.1 ± 6.3
Fumarate	75.0 ± 9.7
Succinate	124 ± 12.3

Enzyme activities were measured at pH 7.3 and 30 °C in the presence of 0.5 mM PEP. The concentration of each metabolite was 1 mM, except for GTP (5 mM), acetyl-CoA (0.4 mM), and fructose-1,6-bisphosphate (2 mM). Mock indicates the enzymatic activity in the absence of additional compounds. Data represent means ± SD from three independent assays. Mock was set at 100%.