Table 2. Σ values for the viral RdRp sequence motifs.

Motif	ϕ12	Apo	Mg2+	Mg2+/GMPCPP	ts-mutant	ϕ6	Apo	Mg2+
F	V299, γ1/2	0.20	0.27	0.22	0.28
F	V299, γ2/1	0.24	0.29	0.26
A	V350, γ1/2	0.77	0.60		0.62
A	V350, γ2/1			0.74
B	I425, δ1				0.00
B	L427, δ1/2	0.67	0.54	0.56	0.55
B	L427, δ2/1	0.85	0.76	0.68	0.72
B	V431, γ1/2	0.60	0.54	0.62	0.67	I408, δ1	0.72	0.75
B	V431, γ2/1		0.62	0.68
B	V434, γ1/2		0.65	0.70	0.56
B	V434, γ2/1	0.62	0.81	0.75	0.75
B	I435, δ1	0.80	0.74	0.77
C						I446, δ1	0.92	0.88
C	I465, δ1	0.48	0.64	0.65	0.68	I449, δ1	0.80	0.80
C	I472, δ1	0.64	0.71	0.62	0.78
C	V473, γ1/2	0.14	0.09	0.06	0.17
D	L487, δ1/2	0.13		0.17	0.24
D	L488, δ1/2	0.16
D	L488, δ2/1	0.10	0.12	0.07	0.22
D	M497, ε1	0.65	0.36	0.36	0.46
D	V500, γ1/2	0.55	0.48	0.43	0.46	I488, δ1	0.04	0.08
E						I500, δ1	0.03	0.07
E	L515, δ1/2	0.34	0.30	0.25	0.39

The corresponding residues for ϕ6 and ϕ12 P2 in the table have been aligned based on their sequential position on their respective motifs (below; probe methyls have been indicated by the green font. The conserved residues are shown in bold font. I425 (italicized) that exists only for the ts-mutant (T425 in wild-type) is the most dynamic of all Ile residues in the mutant.

Motif A

ϕ12: ³⁴⁹D SSYDHSFSE³⁵⁹

ϕ6: ³²⁴DVSDHD-TFWPG³³⁴

Motif B

ϕ12: ⁴¹⁶AGNRSGHAFTS FAK WK D⁴³⁶

ϕ6: ³⁹³SGQGATDLMGTLLMS TYLVM⁴¹³

Motif C

ϕ12: ⁴⁶¹PFGC NNGDDE WFKS⁴⁷⁷

ϕ6: ⁴⁴⁵E RQ SKSDDAMLGWTK⁴⁶¹

Motif D

ϕ12: ⁴⁸⁷ ETQPQEQR FK ⁵⁰⁰

ϕ6: ⁴⁷⁵LKEGKVNPSPYMK ⁴⁸⁸

Motif E

ϕ12: ⁵⁰⁵GAVFSGSVYQ IG⁵¹⁷

ϕ6: ⁴⁹³GGAFLGD LLYDS⁵⁰⁵

Motif F

ϕ12: ²⁹⁵RTR ²⁹⁹

ϕ6: ²⁶⁸RRRTA²⁷²