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. 2017 Jan 23;17:94. doi: 10.1186/s12879-017-2205-3

Fig. 5.

Fig. 5

The microarrays were validated by using PCR reactions. Panel a depicts up-regulated genes and panel b down-regulated genes. Lane 1 represents the corresponding PCR amplification of the indicated gene from untreated mice and lane 2 represents the corresponding PCR amplification of the indicated gene from the immunised mouse group. Each mouse’s RNA was pooled and used for RT-PCR and the genes for validation were randomly assigned. The results are representative of three individual experiments. MWM: Molecular Weight Marker