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. 2016 Dec 28;15(Suppl 2):130. doi: 10.1186/s12938-016-0263-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Expression of Cdc42 in WT- and Sil-HaCaT cells in four migration patterns at different time points. a, b IF images and time courses of Cdc42 expression in WT-HaCaT cells (a). Data were presented as the mean ± SE of normalized FI of totally >9 cells at the leading edge. ^††The level of statistical significance of difference in normalized mean FI at t = 1 h between C/S IN and C/S OUT patterns in WT-HaCaT cells (b). c, d IF images and time courses of Cdc42 expression in Sil-HaCaT cells (c). Data were presented as the mean ± SE of normalized FI of totally >9 cells at the leading edge (d). e Optical images of pseudopodium formation in WT-HaCaT cells. Arrows indicate the pseudopodium at day 2. Scale bar 50 μm