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. 2017 Jan 24;14:4. doi: 10.1186/s12977-017-0328-7

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Biochemical GTPase activation assay reveals HIV significantly activates Rac1 and Cdc42. Primary CD4+ T cells were exposed to CCR5-tropic HIV-1 or microvesicles for 1 min and then assessed for RhoA, Rac1, or Cdc42 activation using a small GTPase activation assay (G-LISA). Results were normalized to the microvesicle controls to determine fold-changes in activation. The mean and SD of 6 independent experiments are shown