Fig. 2.

miR-939 increases cellular sensitivity to 5-Fu in vitro and in vivo. a concentration-dependent growth inhibition in response to 5-Fu in miR-939-overexpressing, miR-939-silenced, and control GC cells. b quantification of the colony formation efficiency in miR-939-overexpressing, miR-939-silenced, and control GC cells treated with 5-Fu chemotherapy. c miR-939 sensitizes GC cells to 5-Fu-induced apoptosis. miR-939-overexpressing and vector control cells treated with indicated dose of 5-Fu for 24 h. Cell apoptotic death events were monitored by Annexin V/PI staining and flow cytometry assays. d after treatment, cleaved caspase 3 and PARP levels were determined by western blot, and GAPDH was used as a normalized control. The quantitative values under the bands were the relative ratios cleaved PARP, cleaved caspase-3 to GAPDH from densitometric analysis. e the same amount (3x10⁶) of miR-939-overexpressing and control GC cells were injected subcutaneously into the flanks of nude mice. Starting on day 7, the mice were given 5-Fu or DMSO intraperitoneally. f, g and h the tumor volumes (f) and mice weight (h) were monitored on day 7 and then every 2 days, as indicated, and the xenografts were excised and weighed on day 25 (g). *P < 0.05, ** P < 0.01