. 2016 Dec 22;17(Suppl 13):1024. doi: 10.1186/s12864-016-3318-6

Table 4.

PICRUSt showing predicted relative abundance of KEGG ortholog groups (Level 2 KOs)

KEGG pathways	NH frogs			AH frogs
KEGG pathways	Fall	Winter	Spring	4 °C
Amino acid metabolism	9.169 ± 0.301^a	9.222 ± 0.337^a	9.084 ± 0.215^a	10.855 ± 1.291^b
Cell motility	4.297 ± 0.552^a	4.338 ± 0.508^a	4.674 ± 0.238^a	3.258 ± 0.3^b
Cellular processes and signaling	3.572 ± 0.107	3.64 ± 0.135	3.702 ± 0.123^a	3.488 ± 0.102^b
Circulatory system	0.005 ± 0.004	0.004 ± 0.003^a	0.004 ± 0.005	0.025 ± 0.026^b
Endocrine system	0.265 ± 0.018^a	0.273 ± 0.026^a	0.258 ± 0.02^a	0.463 ± 0.196^b
Environmental adaptation	0.165 ± 0.013^a	0.169 ± 0.013^a	0.172 ± 0.012^a	0.149 ± 0.004^b
Enzyme families	2.224 ± 0.059^a	2.216 ± 0.052	2.155 ± 0.128	1.946 ± 0.391^b
Folding, sorting and degradation	2.092 ± 0.107^a	2.162 ± 0.173^a	2.095 ± 0.142^a	2.384 ± 0.065^b
Genetic information processing	2.404 ± 0.081	2.472 ± 0.118	2.507 ± 0.121^a	2.321 ± 0.072^b
Immune system diseases	0.03 ± 0.006^a,c	0.029 ± 0.006^a,c	0.024 ± 0.005^b	0.036 ± 0.007^c
Infectious diseases	0.312 ± 0.013^a	0.318 ± 0.016^a	0.319 ± 0.031	0.394 ± 0.055^b
Lipid metabolism	2.759 ± 0.107^a	2.733 ± 0.128^a	2.71 ± 0.112^a	3.96 ± 1.373^b
Membrane transport	14.685 ± 1.045^a	14.072 ± 1.519^a	14.645 ± 1.177^a	9.828 ± 2.509^b
Metabolism	2.492 ± 0.057^a	2.505 ± 0.068^a	2.492 ± 0.065^a	2.616 ± 0.102^b
Metabolism of other amino acids	1.479 ± 0.08^a	1.463 ± 0.077^a	1.442 ± 0.107^a	2.042 ± 0.516^b
Metabolism of terpenoids and polyketides	1.353 ± 0.115^a	1.384 ± 0.142^a	1.351 ± 0.134^a	2.282 ± 0.884^b
Nervous system	0.093 ± 0.003^a	0.092 ± 0.003^a	0.091 ± 0.003^a	0.104 ± 0.008^b
Poorly characterized	4.872 ± 0.083^a	4.909 ± 0.15	4.912 ± 0.135	5.216 ± 0.403^b
Signal transduction	2.15 ± 0.171^a	2.194 ± 0.18^a,b,d	2.302 ± 0.126^b	1.926 ± 0.111^c
Transcription	2.875 ± 0.159^a	2.765 ± 0.248^a	2.834 ± 0.262^a	2.193 ± 0.342^b
Xenobiotics biodegradation and metabolism	1.75 ± 0.152^a	1.709 ± 0.177^a	1.789 ± 0.306^a	3.463 ± 2.176^b

Abbreviations: AH frogs artificially hibernating frogs, NH frogs, nonhibernating frogs. Values are means ± SD. Within each row, values not sharing superscripts (a, b, c, and d) differ significantly (p-value < 0.05, Wilcoxon’s test)