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. 2017 Jan 24;8:14. doi: 10.1186/s13287-016-0465-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Immunophenotypic analysis of CB-MSC. a Characterization of LL-CBMSC (n = 5) by flow cytometry using a panel of 14 cell surface markers. Boxes extend from 25^th percentile to the 75^th percentile, the middle line represents median value and the whiskers extend from minimum to maximum values. Data are displayed as rMFI on the unstained control. b-e Phenotypic modifications induced on LL-CBMSC (n = 4) by inflammatory stimuli, i.e., treatment with 10 ng/ml IFN-γ-1b and 15 ng/ml TNF-α for 48 hours before staining with the appropriate mAb combination. Data are expressed as rMFI with respect to the FMO control. P values <0.05 were considered statistically significant. Abbreviations: rMFI relative median fluorescence intensity, FMO fluorescence-minus-one, mAb monoclonal antibody