. 2017 Jan 24;14:12. doi: 10.1186/s12985-016-0679-z

Table 1.

Primers, annealing temperatures and amplicons used in PCR assays for SNPs in the TLR2 gene

Gene	GenBank accession no.^a	SNP^b name	Primer sequences (5′-3′)		Annealing temperature [°C]	Amplicon length (bps)^c
TLR2	NC_000004.12	1350 T>C	External	For: AATTCAGCCTGTGAGGATGC	52	361
		(rs3804100)	External	Rev: GTAAGAGGGAGGCATCTGGTA	52	361
		(Ser450)	Internal	For: TCATTTGGCATCATTGGAAA	55	248
		(S450)	Internal	Rev: GAGTTGCGGCAAATTCAAAG	55	248
		2029 C>T; 2258 G>A	External	For: CGGAATGTCACAGGACAGC	52	605
		(rs121917864; rs5743708)	External	Rev: GGACTTTATCGCAGCTCTCAG	52	605
		(Arg677Trp; Arg753Gln)	Internal	For: GCCTACTGGGTGGAGAACCT	59	340
		(R677W; R753Q)	Internal	Rev: GGCCACTCCAGGTAGGTCTT	59	340

^a No. number

^b SNP single nucleotide polymorphism

^c bps base pairs