Fig. 6.

Rab32-mediated neuronal killing assay. a HFNs were transfected with pIRES2-EGFP, or pIRES2-EGFP expressing Flag-tagged Rab32WT, Rab32Q85L, and Rab32T39N as well as the mCherry reporter-tagged shRab32. After 24, 48, and 72 h, neurons were fixed and analyzed under a fluorescent microscopy. Note: the red color of mCherry was converted to green for the sake of consistency with the rest of the micrographs. Scale bar 30 μm. b Rab32-mediated neuronal killing was evaluated at 48 and 72 h post-transfection. c SH-SY5Y cells were transfected with pIRES2-EGFP, or pIRES2-EGFP expressing Flag-tagged Rab32WT, Rab32Q85L, and Rab32T39N as well as the mCherry reporter-tagged shRab32. After 24 h in culture, the cells were fixed, and the percentage of surviving neurons in comparison to the control (EGFP) was analyzed. n = 3; *p < 0.05; **p < 0.01; ***p < 0.001