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. 2016 Dec 22;17(Suppl 13):1029. doi: 10.1186/s12864-016-3322-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effects of POLA2 gene silencing. a Protein expression after knockdown of POLA2 by siRNA. Comparison of POLA2 protein expression in H1299 cell line by Western blotting after 48 and 72 h of siRNA transfection. Internal control for equal loading: glyceraldeyde phosphate dehydrogenase (GAPDH). b Chemosensitivity assay by comparing IC₅₀ values before and after knockdown of POLA2. Results indicated that the H1299 cell line became more resistant to gemcitabine treatment after siRNA knockdown of POLA2 (Knockdown), as compared to the non-transfected H1299 cell line having wild-type POLA2 (Control). Experiments were conducted in triplicates and repeated at least 3 times. Data are shown as the mean ± SD (*p <0.05)