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. 2017 Jan 23;17:21. doi: 10.1186/s12870-017-0978-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Karyological characterization of Atropa belladonna (accessions 986 and 987). a and b Double fluorescent staining with 4′,6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA), respectively; c Localization of 5S and 45S rDNA sequences on chromosomes; The chromosomes were stained by DAPI (blue fluorescence), hybridization signals of 5S (red) and 45S rDNA (green) are marked by arrows; d Active 45S rDNA (nucleolar organizing region, NOR) sites (arrows) in Atropa belladonna detected by silver staining; Scale bar, 10 μm