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. 2017 Jan 23;17:21. doi: 10.1186/s12870-017-0978-6

Table 1.

Number of base substitutions in 5S rDNA of Atropa belladonna

Clone Length Transversions Transitions Total number of base substitutions
C → T, G → A T → C, A → G
Group I: long repeats
 Ab-5S-05 199 0 1 0 1
 Ab-5S-09 199 1 1 1 3
 Ab-5S-14 199 2 2 0 4
 Ab-5S-15 199 0 0 0 0
 Ab-5S-16 199 2 2 0 4
 Ab-5S-24 200 5 3 0 8
 Ab-5S-93 198 0 0 0 0
Total for group I 1393 10 (50.0%) 9 (45.0%) 1 (5.0%) 20 (100%)
Group II: intermediate repeats
 Ab-5S-03 112 0 0 0 0
 Ab-5S-36 144 6 9 1 16
 Ab-5S-37 112 0 1 0 1
 Ab-5S-43 112 0 0 0 0
 Ab-5S-53 112 0 0 0 0
 Ab-5S-52 112 1 2 0 3
 Ab-5S-54 112 0 0 0 0
 Ab-5S-61 112 0 1 0 1
Total for group II 928 7 (33.3%) 13 (61.9%) 1 (4.8%) 21 (100%)
Group III: short repeats
 Ab-5S-02 54 1 5 0 6
 Ab-5S-32 53 1 5 0 6
 Ab-5S-33 54 1 4 0 5
 Ab-5S-39 62 3 4 0 7
 Ab-5S-42 54 1 2 0 3
Total for group III 277 7 (25.9%) 20 (74.1%) 0 27 (100%)

Note: The number of base substitutions was calculated comparing sequences of individual clones and the consensus sequence. Lengths of clones are presented without primers used for PCR