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. Author manuscript; available in PMC: 2017 Jan 24.
Published in final edited form as: Nat Protoc. 2016 Oct 27;11(12):2328–2356. doi: 10.1038/nprot.2016.114

Figure 6.

Figure 6

T-REX targeting is equally efficient in human (HEK-293) cells and E. coli. Halo-Keap1 human protein55–57 is used as a model protein. Coomassie-stained membrane is used to evaluate uniform loading of total lysate proteins in each lane. Independent duplicates of Keap1 targeting results are presented in a. Partial cleavage of the fusion protein (into separate Halo and Keap1 proteins) was often observed during standard E. coli growth conditions, accounting for the observed Cy5 signal on the Halo band (see no-light, no-TEV sample lane in b). Theoretical MWs: Halo, ~33 kDa; Keap1, ~70 kDa. L, MW ladder; TEV, TEV protease.