Skip to main content
. Author manuscript; available in PMC: 2017 Jan 24.
Published in final edited form as: Nat Protoc. 2016 Oct 27;11(12):2328–2356. doi: 10.1038/nprot.2016.114

Figure 8.

Figure 8

Immunofluorescence analysis of endogenous PIP3 phosphoinositide in fixed cells subsequent to PTEN-targeted redox modification enabled by T-REX in live cells. Live HEK-293 cells expressing Halo-PTEN were subjected to T-REX-targeted HNEylation of PTEN55. Dominant loss-of-function inactivation of PTEN results in upregulation of PIP3. The cells were fixed and immunostained by anti-PIP3 (green) and anti-Halo (red). Error bars represent the standard error of the mean (N = 86). Scale bars, 20 µm. Inset: schematic of the PI3K/PTEN signaling. Partial inactivation of PTEN raises the levels of PIP3. ****, P < 0.0001.