Figure 1.

PAR1 mRNA detection in humans and mice. (A and B) PAR₁ mRNA detection in colonic biopsies from control (normal), ulcerative colitis (UC), or Crohn disease (CD) patients, by RT-PCR (A) and real-time RT-PCR (B). A minimum of 5 patients per group was considered for quantification, and representative RT-PCR for 3 patients per group is shown (blots). hPAR₁, human PAR₁. (C) PAR₁ mRNA detection in colonic tissues from naive mice or mice with TNBS- or DSS-induced colitis. PAR₁ mRNA detection was assessed by the amplification of a specific PCR fragment and was expressed as percentage of amplified GAPDH fragment. For quantification, values are mean ± SEM; n = 5 per group. *P < 0.05, **P < 0.01, ***P < 0.001.