Figure 8.

TNF-α (A and D), IFN-γ (B and D), and IL-12 (D) protein expression, and IFN-γ, TNF-α, IL-2, COX-1, COX-2, constitutive NO synthase (cNOS), iNOS, and β-actin mRNA levels (C), in the colon of BALB/c naive mice, TNBS- and PAR₁ antagonist–treated mice, or TNBS- and vehicle-treated mice (A–C), or in the colon of BALB/c naive mice, mice treated with DSS plus vehicle, or mice treated with DSS plus PAR₁ antagonist (D). For A, B, and D, protein content of the mouse colon was determined by ELISA, and values are mean ± SEM; n = 12 per group. *Significantly different from naive group; **significantly different from vehicle-treated group. For C, RT-PCR mRNA amplification was representative of 12 mice per group for each mediator.