Figure 9.

In vitro release of TNF-α (A) and IFN-γ (B and C) by lamina propria monocytic cells that were left unstimulated (Alone) or stimulated with CD3 and CD28 alone, CD3 and CD28 plus PAR₁-activating peptide (PAR₁-AP), or PAR₁-activating peptide alone. Cells were isolated from naive (control) mice (A–C) or were isolated 7 days after intracolonic administration of TNBS from mice that had been treated with the PAR₁ antagonist (A and B) or with vehicle (A–C). Values are mean ± SEM; n = 12 per group. *Significantly different from control group in A and B, and significantly different from medium alone in C; **significantly different from vehicle-treated group in A and B, and from naive group in C; ^ψsignificantly different from medium alone of cells from TNBS-treated mice.