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. 2016 Oct 14;24(1):132–143. doi: 10.1038/cdd.2016.108

Figure 1.

Figure 1

Effects of TM7SF3 on caspase activity in human islets and other cell types. Dispersed human islets (a), MIN6 (b), U2-OS (c–e) and HEK cells (f) were transfected with the indicated plasmids (harboring the cDNA of TM7SF3 or GFP alone) or siRNAs for 48 h (a, b and f) or for 8 days (by triple transfections) (c–e). Cells remained untreated (open bars) or were treated with Cytomix ‘3X' for 16 h (black bars), followed by assay of caspase activity (a, b and d) and cell number (Crystal Violet) (e). Alternatively, total cell extracts were resolved by SDS-PAGE and immunoblotted with the indicated antibodies (c). Data are the mean±S.E.M. of at least three independent experiments in four replicates. *P<0.05; **P<0.01; ***P<0.001