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. 2016 Oct 14;24(1):132–143. doi: 10.1038/cdd.2016.108

Figure 2.

Figure 2

Effects of TM7SF3-siRNA on iNOS mRNA levels and NO production. Dispersed human islets (a), MIN6 (b and c), HepG2 (d) and U2-OS (e) cells were transfected for 48 h (a–d) or 6 days (e) with TM7SF3-siRNA or with a non-targeting sequence (control). Human islets (a) were then treated with thapsigargin (Thap; 100 nM) for 16 h or were left untreated. MIN6 cells (b and c) were treated with Cytomix ‘1X' for 12 h. HepG2 (d) and U2-OS (e) cells remained untreated. RNA was extracted from all cells and quantified by qRT-PCR (a, b, d and e). Alternatively, the medium was collected, and the concentration of nitrite was measured by Griess assay (c). Bar graphs are the mean±S.E.M. of three independent experiments in duplicates. *P<0.05; ***P<0.001 versus non-targeting siRNA assayed under the same conditions. Results in a were obtained in three (out of four batches) of human islets