Skip to main content
. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Mech Behav Biomed Mater. 2016 Aug 15;65:383–397. doi: 10.1016/j.jmbbm.2016.08.010

Figure 2.

Figure 2

Effects of actomyosin contraction on early forebrain morphogenesis (HH11–12). Stage HH11 embryos were cultured 6 h in media containing blebbistatin or calyculin A to inhibit or enhance contractility, respectively (control n=9, blebbistatin n=15, calyculin A n=12). (A–C) Bright-field images reveal altered BT morphology under each condition (dorsal view). White arrowheads indicate AIS, black arrowheads indicate the optic stalk (OS), and white arrows highlight previously formed DMB. Dashed lines indicate locations used to compute average radii for T (red), D (green) and M (blue). Scale bars: 200 µm. (A’–C’) Representative OCT cross sections of SP for each case. Dashed white lines indicate locations used to compute average radii for the OS, and the average radius of T was calculated considering lumen area between the optic stalks. Scale bars: 100 µm. (A”–C”) Representative AIS cross sections for each case. Scale bars: 100µm. (D) Relative constrictions based on average radii at locations indicated in (A–C), as well as relative OS stretch ratio λOS based on locations indicated in (A’–C’). *P<0.05, **P<0.001 (E) Representative sagittal cross sections before and after 6 h in blebbistatin, followed by washout (n=6). White arrowheads indicate AIS. Scale bars: 200 µm.