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. Author manuscript; available in PMC: 2017 Jan 24.
Published in final edited form as: Biochem J. 2016 May 24;473(14):2219–2224. doi: 10.1042/BCJ20160339

Figure 1. Cpx splits the C-terminal region of a single SNAREpin in the nanodisc sandwich.

Figure 1

(a) Nanodisc sandwich harbouring a single SNAREpin with the FRET pair at the N-terminal region (NN). VAMP2 Q33C–Cy3 and Syn1A I203C–Cy5 are used for NN. The majority of the population is distributed in the high FRET region. (b) Cpx at 2 μM does not affect the FRET distribution of NN. (c) Nanodisc sandwich harbouring a SNAREpin with the FRET pair at the C-terminal region (CC). VAMP2 A72C–Cy3 and Syn1A V241C–Cy5 were used to make CC. The distribution shows two distinct populations in the high and in the low FRET regions, but little in the mid FRET region. (d) Cpx at 2 μM pulls both the low and high FRET populations towards the mid FRET region. All experiments were independently conducted at least four times. Total of 499, 446, 502 and 524 traces were analysed for (a)–(c) and (d) respectively.