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. Author manuscript; available in PMC: 2017 Jan 24.
Published in final edited form as: Biochem J. 2016 May 24;473(14):2219–2224. doi: 10.1042/BCJ20160339

Figure 4. The N-terminal region of Cpx plays a role in restructuring the trans-SNAREpin.

Figure 4

Both N-terminal Cpx mutants (a) M5E/K6E and (b) Cpx-(27–134) can pull the high FRET population down to mid FRET for CC. However, they are incapable of pushing the low FRET population to the mid FRET region. All experiments were independently conducted four times. Total of 704 and 494 traces were analysed for (a) and (b) respectively.