Figure 6.

Hesperetin-mediated frequency-dependent Nav1.5 channel blockage. (A) Thirty repetitive 50-ms pulses causing depolarization from a holding potential of −120 mV to −30 mV were applied at three different frequencies, 2, 5, and 10 Hz, in the absence or presence of 100 μmol/L hesperetin. (B) The amplitudes of the currents achieved during each pulse were normalized to the amplitudes of the currents acquired during the first pulse and then plotted against pulse numbers (n=11). ^**P<0.01 vs corresponding control. (C) Hesperetin (100 μmol/L)-induced changes (%) in current amplitude are plotted against pulse numbers (n=8). Data are expressed as the mean±SEM. ^*P<0.05, ^**P<0.01 vs 2 Hz. HSP: hesperetin.