Figure 1.

Physcion inhibits cell proliferation and induces G₁ phase arrest in human nasopharyngeal carcinoma cell line CNE2. (A) The reduction in cell viability by physcion was assessed using MTT assays following treatment with physcion at different dosages for 24 and 48 h. (B) The anti-proliferative effect was determined by colony formation assays following treatment with physcion. (C) Physcion blocks cell cycle progression as determined by flow cytometry following treatment with physcion at the indicated concentrations for 48 h. (D) Physcion modulated cell cycle-regulated proteins as assessed by Western blotting following treatment with physcion at the indicated concentrations for 48 h. Mean±SD. ^*P<0.05, ^**P<0.01 vs vehicle.