. Author manuscript; available in PMC: 2018 Feb 15.

Published in final edited form as: Anal Chim Acta. 2016 Dec 10;954:140–150. doi: 10.1016/j.aca.2016.12.003

Table 1.

Gradient used in the first-dimension mixed-mode liquid chromatography. A: heptane, B: acetone, C: chloroform:methanol:ammonia solution (80:19.5:0.5, v/v/v) and D: chloroform:methanol:TEA:water (69.53:25.58:0.49:4.40, v/v/v/v).

Time (min)	%A	%B	%C	%D	Flow Rate (mL min⁻¹)
0	94	6	0	0	1.0
9	85	15	0	0	1.0
11	80	20	0	0	1.0
12	80	20	0	0	1.0
17	0	0	100	0	1.0
22	0	0	100	0	1.0
27	0	0	60	40	1.0
29	0	0	60	40	1.0
42	0	0	0	100	1.0
49	0	0	0	100	1.0
51	0	0	100	0	5.0
54	0	0	100	0	5.0
55	94	6	0	0	5.0
58	94	6	0	0	5.0

Time (min)	%A	%B	%C	%D	Flow Rate (mL min⁻¹)
0	94	6	0	0	1.0
9	85	15	0	0	1.0
11	80	20	0	0	1.0
12	80	20	0	0	1.0
17	0	0	100	0	1.0
22	0	0	100	0	1.0
27	0	0	60	40	1.0
29	0	0	60	40	1.0
42	0	0	0	100	1.0
49	0	0	0	100	1.0
51	0	0	100	0	5.0
54	0	0	100	0	5.0
55	94	6	0	0	5.0
58	94	6	0	0	5.0

Time (min)	%A	%B	%C	%D	Flow Rate (mL min⁻¹)
0	94	6	0	0	1.0
9	85	15	0	0	1.0
11	80	20	0	0	1.0
12	80	20	0	0	1.0
17	0	0	100	0	1.0
22	0	0	100	0	1.0
27	0	0	60	40	1.0
29	0	0	60	40	1.0
42	0	0	0	100	1.0
49	0	0	0	100	1.0
51	0	0	100	0	5.0
54	0	0	100	0	5.0
55	94	6	0	0	5.0
58	94	6	0	0	5.0

Time (min)	%A	%B	%C	%D	Flow Rate (mL min⁻¹)
0	94	6	0	0	1.0
9	85	15	0	0	1.0
11	80	20	0	0	1.0
12	80	20	0	0	1.0
17	0	0	100	0	1.0
22	0	0	100	0	1.0
27	0	0	60	40	1.0
29	0	0	60	40	1.0
42	0	0	0	100	1.0
49	0	0	0	100	1.0
51	0	0	100	0	5.0
54	0	0	100	0	5.0
55	94	6	0	0	5.0
58	94	6	0	0	5.0