Table 2.
Different lipid classes resolved by mixed-mode LC-ELSD using a monolithic silica column and their retention times (n = 5) with standard deviations (S.D.) and relative standard deviations (R.S.D.). The fraction where they were collected (A-M) is also specified.
| Lipid Class | Fraction | Retention time (min) | S.D. | R.S.D. (%) |
|---|---|---|---|---|
| CE | A | 1.69 | 0.01 | 0.80 |
| TG | A | 2.09 | 0.05 | 2.48 |
|
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| Cholesterol | B | 3.86 | 0.03 | 0.74 |
| 1,3-DG | B | 4.01 | 0.05 | 1.36 |
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| 1,2-DG | C | 4.81 | 0.08 | 1.66 |
|
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| 1-MG | D | 13.01 | 0.22 | 1.69 |
|
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| Cer | E | 14.37 | 0.23 | 1.57 |
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| FA C26:0 | F | 17.50 | 0.26 | 1.48 |
| FA C18:1 | F | 17.88 | 0.24 | 1.32 |
| FA C12:0 | F | 18.25 | 0.22 | 1.22 |
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| PG | G | 22.06 | 0.03 | 0.14 |
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| CL | H | 24.13 | 0.06 | 0.25 |
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| PE | I | 28.14 | 0.02 | 0.08 |
| pPE | I | 28.43 | 0.02 | 0.06 |
| LysoPG | I | 29.24 | 0.03 | 0.11 |
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| PI | J | 30.38 | 0.03 | 0.11 |
| PS | J | 31.69 | 0.06 | 0.20 |
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| LysoPE | K | 32.69 | 0.07 | 0.23 |
| PC | K | 33.37 | 0.04 | 0.11 |
| pPC | K | 33.70 | 0.03 | 0.09 |
| PA | K | 34.68 | 0.02 | 0.05 |
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| SM | L | 38.42 | 0.01 | 0.03 |
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| sn2-LysoPC | M | 41.35 | 0.01 | 0.01 |
| sn1-LysoPC | M | 41.77 | 0.02 | 0.04 |
CE: cholesterol ester; TG: triacylglycerol; 1,3-DG: 1,3- diacylglycerol; 1,2-DG: 1,2- diacylglycerol; 1-MG: 1-monoacylglycerol; Cer: ceramide; FA: free fatty acid; PG: phosphatidylglycerol; CL: cardiolipin; PE: phosphatidylethanolamine; pPE: phosphatidylethanolamine plasmalogen; LysoPG: lysophosphatidylglycerol; PI: phosphatidylinositol; PS: phosphatidylserine; LysoPE: lysophosphatidylethanolamine; PC: phosphatidylcholine; pPC: phosphatidylcholine plasmalogen; PA: phosphatidic acid; SM: sphingomyelin; sn2-LysoPC: sn2-lysophosphatidylcholine; sn1-LysoPC: sn1-lysophosphatidylcholine.