Figure 4.

Adiponectin alleviated adipocyte ER stress and reduced cytosolic Ca²⁺. Adipocytes were first incubated with TM; recombinant murine adiponectin was then added to the medium at 10 μg/ml. n=3 for each treatment. (a) Relative AdipoR1 level in adipocytes. (b) Protein levels of ER stress and apoptosis. (c) Cytosolic Ca²⁺ in adipocytes. (d) Flow cytometry (FCM) analysis of Cytosolic Ca²⁺ in Figure 4c. (e) FFA level in adipocytes. (f) Protein levels of FATP1, CPT-1, PGC-1-α, Bax and Bcl-2 in adipocytes measured by using ELISA method. Values are means±S.D. *P<0.05 compared with the control group