Figure 5.

Adiponectin blocked PA-induced apoptosis and decreased ATF2 in adipocytes. Recombinant murine adiponectin was added in the medium at 10 μg/ml. n=3 for each treatment. (a) Cell viability of adipocytes treated with palmitic (200 μg/ml, PA) for up to 48 h. (b) Relative mRNA levels of adiponectin and AdipoR1 in adipocytes incubated with PA for 24 h. (c) Hoechst and Red Oil O-stained adipocytes incubated with PA for 24 h. (d) Relative mRNA levels of Chop, ATF2, caspase 9 and caspase 3 in adipocytes incubated with PA for 24 h. (e) Hoechst-stained adipocytes incubated with PA, followed with adiponectin (10 μg/ml). (f) Protein levels of PPARα, ATF2, Chop, cleaved-caspase 9 and cleaved-caspase 3 in adipocytes incubated with PA, followed with adiponectin (10 μg/ml). Values are means±S.D. *P<0.05 compared with the control group