Figure 6.

Adiponectin inhibited mitochondrial apoptosis pathway and reduced ER stress in adipocyte. Adipocytes were infected with recombinant vectors of adiponectin (pAd-APN or si-APN) for 48 h. n=3 for each treatment. (a) Protein levels of adiponectin and adipoR1 in adipocytes. (b) FFA concentration in adipocytes. (c) Cytosolic Ca²⁺ detected by using Fluo-3-AM stain and flow cytometry (FCM) analysis of fluorescence intensity in adipocytes. (d) Annexin V-FITC/PI double staining and flow cytometry analysis of adipocyte apoptosis stages. (e) JC-1 staining and flow cytometry analysis of mitochondrial membrane potential in adipocytes. (f) Cyt C immunofluorescence in adipocytes. (g) Protein levels of ATF2 and apoptosis genes in adipocytes. pAd-APN, recombinant adenovirus overexpression vector of adiponectin; si-APN, recombinant lentiviral interference vector of adiponectin. Values are means±S.D. *P<0.05 compared with the control group