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. 2016 Nov 24;7(11):e2488. doi: 10.1038/cddis.2016.385

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Molar ratios of SM and Cer are reduced in Golgi-lipidome of smpd3−/− chondrocytes. MS/MS profiling of (a-h) species of SM (a), dihydroceramide (DH-Cer) (b), Cer (c), PC (d), DAG (e), phosphatidylserine (PS) (f), PI (g) and phosphatidylethanolamine (PE) (h) of Golgi fraction of control and smpd3−/− chondrocytes. (i) Molar ratio of main PL classes (PC, PI and PE) and SM, Cer, C and DAG. (j and k) Thin layer chromatographic separation of lipid extract of control and smpd3−/− chondrocytes. Solvent system: cyclohexane/ethylacetate 3:2 v/v (j); two runs in chloroform/ethanol/water/triethylamine 30:35:7:35 v/v/v (k). N=3