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. 2016 Nov 24;7(11):e2486. doi: 10.1038/cddis.2016.383

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression of EZH2 in human colon cancer tissues. (a) IHC staining of EZH2 in both tumoral (left) and adjacent (right) tissues from two representative samples on the tissue array. Different magnification from the same sample was shown (scale bar: 100 μm). (b) Based on the EZH2 staining intensity, five groups were classified and plotted from the weakest (±) to the strongest (++++) in both tumoral and adjacent tissues on the tissue array (n=90), as described in Materials and methods section. (c) High EZH2 expression was correlated with TNM stage (III/IV versus I/II) (P<0.05) in the samples according to their clinicopathologic features listed in Supplementary Table 1. (d) IB analysis of EZH2 expression in eight pairs of colon cancer and adjacent normal tissues from patients. GAPDH was used as a loading control. Quantification of the protein expression in the tumor (T) and adjacent tissues (A) was analyzed on the right (n=8) (***P<0.001)