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. 2016 Nov 24;7(11):e2486. doi: 10.1038/cddis.2016.383

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Overexpression of EZH2 decreased the DNA damage response caused by LDM. (a) Expression of EZH2 in wild-type and EZH2 +/+ cells as in Figure 6 were determined by IB analysis. (b) EZH2 overexpression decreased LDM-induced γ-H2AX level in both cell lines. Wild-type and EZH2 +/+ cell lines were seeded in six-well plates, and treated with LDM at 0.5 nM for 72 h, and then γ-H2AX level was analyzed by FACS as described in Materials and methods section. (c) Quantitation of the FACS analysis of three independent experiments (*P<0.05)